two-step omniscript reverse transcription kit (Qiagen)
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two-step omniscript reverse transcription kit
Two Step Omniscript Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-step+omniscript+reverse+transcription+kit/two+step+omniscript+reverse+transcription+kit/pm22009262-72-9-14
Average 90 stars, based on 1 article reviews
Two Step Omniscript Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-step+omniscript+reverse+transcription+kit/two+step+omniscript+reverse+transcription+kit/pm22009262-72-9-14
Average 90 stars, based on 1 article reviews
two-step omniscript reverse transcription kit - by Bioz Stars,
2026-10
90/100 stars
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Extraction:Article Title: Novel active kinetoplastids associated with hypersaline anoxic basins in the Eastern Mediterranean deep-sea Article Snippet: The combination of nearly saturated salt concentration and corresponding high density, high hydrostatic pressure, absence of light, anoxia, and a sharp chemocline make the deep hypersaline anoxic basins in the Eastern Mediterranean Sea some of the most polyextreme habitats on Earth.. Using kinetoplastid-specific primers, we detected kinetoplastid flagellates in some of the harshest deep-sea environments known to date, including some whose small subunit ribosomal RNA gene sequences are not closely related to cultured representatives.. Kinetoplastids, including presumably novel representatives appear to be specialists of halocline environments in the Eastern Mediterranean, and to comprise a significant fraction of the protist communities in the brines and haloclines of several basins. Article Title: Microbial eukaryote life in the new hypersaline deep-sea basin Thetis. Article Snippet: Only recently, a novel anoxic hypersaline (thalassic) basin in the eastern Mediterranean was discovered at a depth of 3,258 m. The halite-saturated brine of this polyextreme basin revealed one of the highest salt concentrations ever reported for such an environment (salinity of 348%).. Using a eukaryote-specific probe and fluorescence in situ hybridization, we counted 0.6 9 10 protists per liter of anoxic brine.. SSU rRNA sequence analyses, based on amplification of environmental cDNA identified fungi as the most diverse taxonomic group of eukaryotes in the brine, making deep-sea brines sources of unknown fungal diversity and hotspots for the discovery of novel metabolic pathways and for secondary metabolites. Article Title: A molecular approach to identify active microbes in environmental eukaryote clone libraries. Article Snippet: A rapid method for the simultaneous extraction of RNA and DNA from eukaryote plankton samples was developed in order to discriminate between indigenous active cells and signals from inactive or even dead organisms.. The method was tested using samples from below the chemocline of an anoxic Danish fjord.. The simple protocol yielded RNA and DNA of a purity suitable for amplification by reverse transcription-polymerase chain reaction (RT-PCR) and PCR, respectively. Article Title: Microbial eukaryotes in the hypersaline anoxic L'Atalante deep-sea basin. Article Snippet: Eva Alexander,1 Alexandra Stock,1 Hans-Werner Breiner,1 Anke Behnke,1 John Bunge,2 Michail M. Yakimov3 and Thorsten Stoeck1* University of Kaiserslautern, School of Biology, Erwin-Schroedinger-Strasse 14, D-67773, Kaiserslautern, Germany.. Department of Statistical Science, Cornell University, Ithaca, NY 14853, USA.. Institute for Coastal Marine Environment, IAMC-CNR, Spianata S. Raineri, 86, 98122 Messina, Italy. Amplification:Article Title: Novel active kinetoplastids associated with hypersaline anoxic basins in the Eastern Mediterranean deep-sea Article Snippet: The combination of nearly saturated salt concentration and corresponding high density, high hydrostatic pressure, absence of light, anoxia, and a sharp chemocline make the deep hypersaline anoxic basins in the Eastern Mediterranean Sea some of the most polyextreme habitats on Earth.. Using kinetoplastid-specific primers, we detected kinetoplastid flagellates in some of the harshest deep-sea environments known to date, including some whose small subunit ribosomal RNA gene sequences are not closely related to cultured representatives.. Kinetoplastids, including presumably novel representatives appear to be specialists of halocline environments in the Eastern Mediterranean, and to comprise a significant fraction of the protist communities in the brines and haloclines of several basins. Article Title: Microbial eukaryote life in the new hypersaline deep-sea basin Thetis. Article Snippet: Only recently, a novel anoxic hypersaline (thalassic) basin in the eastern Mediterranean was discovered at a depth of 3,258 m. The halite-saturated brine of this polyextreme basin revealed one of the highest salt concentrations ever reported for such an environment (salinity of 348%).. Using a eukaryote-specific probe and fluorescence in situ hybridization, we counted 0.6 9 10 protists per liter of anoxic brine.. SSU rRNA sequence analyses, based on amplification of environmental cDNA identified fungi as the most diverse taxonomic group of eukaryotes in the brine, making deep-sea brines sources of unknown fungal diversity and hotspots for the discovery of novel metabolic pathways and for secondary metabolites. Article Title: A molecular approach to identify active microbes in environmental eukaryote clone libraries. Article Snippet: A rapid method for the simultaneous extraction of RNA and DNA from eukaryote plankton samples was developed in order to discriminate between indigenous active cells and signals from inactive or even dead organisms.. The method was tested using samples from below the chemocline of an anoxic Danish fjord.. The simple protocol yielded RNA and DNA of a purity suitable for amplification by reverse transcription-polymerase chain reaction (RT-PCR) and PCR, respectively. Article Title: Microbial eukaryotes in the hypersaline anoxic L'Atalante deep-sea basin. Article Snippet: Eva Alexander,1 Alexandra Stock,1 Hans-Werner Breiner,1 Anke Behnke,1 John Bunge,2 Michail M. Yakimov3 and Thorsten Stoeck1* University of Kaiserslautern, School of Biology, Erwin-Schroedinger-Strasse 14, D-67773, Kaiserslautern, Germany.. Department of Statistical Science, Cornell University, Ithaca, NY 14853, USA.. Institute for Coastal Marine Environment, IAMC-CNR, Spianata S. Raineri, 86, 98122 Messina, Italy. cDNA Library Assay:Article Title: Novel active kinetoplastids associated with hypersaline anoxic basins in the Eastern Mediterranean deep-sea Article Snippet: The combination of nearly saturated salt concentration and corresponding high density, high hydrostatic pressure, absence of light, anoxia, and a sharp chemocline make the deep hypersaline anoxic basins in the Eastern Mediterranean Sea some of the most polyextreme habitats on Earth.. Using kinetoplastid-specific primers, we detected kinetoplastid flagellates in some of the harshest deep-sea environments known to date, including some whose small subunit ribosomal RNA gene sequences are not closely related to cultured representatives.. Kinetoplastids, including presumably novel representatives appear to be specialists of halocline environments in the Eastern Mediterranean, and to comprise a significant fraction of the protist communities in the brines and haloclines of several basins. Article Title: Microbial eukaryote life in the new hypersaline deep-sea basin Thetis. Article Snippet: Only recently, a novel anoxic hypersaline (thalassic) basin in the eastern Mediterranean was discovered at a depth of 3,258 m. The halite-saturated brine of this polyextreme basin revealed one of the highest salt concentrations ever reported for such an environment (salinity of 348%).. Using a eukaryote-specific probe and fluorescence in situ hybridization, we counted 0.6 9 10 protists per liter of anoxic brine.. SSU rRNA sequence analyses, based on amplification of environmental cDNA identified fungi as the most diverse taxonomic group of eukaryotes in the brine, making deep-sea brines sources of unknown fungal diversity and hotspots for the discovery of novel metabolic pathways and for secondary metabolites. Article Title: A molecular approach to identify active microbes in environmental eukaryote clone libraries. Article Snippet: A rapid method for the simultaneous extraction of RNA and DNA from eukaryote plankton samples was developed in order to discriminate between indigenous active cells and signals from inactive or even dead organisms.. The method was tested using samples from below the chemocline of an anoxic Danish fjord.. The simple protocol yielded RNA and DNA of a purity suitable for amplification by reverse transcription-polymerase chain reaction (RT-PCR) and PCR, respectively. Article Title: Microbial eukaryotes in the hypersaline anoxic L'Atalante deep-sea basin. Article Snippet: Eva Alexander,1 Alexandra Stock,1 Hans-Werner Breiner,1 Anke Behnke,1 John Bunge,2 Michail M. Yakimov3 and Thorsten Stoeck1* University of Kaiserslautern, School of Biology, Erwin-Schroedinger-Strasse 14, D-67773, Kaiserslautern, Germany.. Department of Statistical Science, Cornell University, Ithaca, NY 14853, USA.. Institute for Coastal Marine Environment, IAMC-CNR, Spianata S. Raineri, 86, 98122 Messina, Italy. Reverse Transcription:Article Title: Novel active kinetoplastids associated with hypersaline anoxic basins in the Eastern Mediterranean deep-sea Article Snippet: The combination of nearly saturated salt concentration and corresponding high density, high hydrostatic pressure, absence of light, anoxia, and a sharp chemocline make the deep hypersaline anoxic basins in the Eastern Mediterranean Sea some of the most polyextreme habitats on Earth.. Using kinetoplastid-specific primers, we detected kinetoplastid flagellates in some of the harshest deep-sea environments known to date, including some whose small subunit ribosomal RNA gene sequences are not closely related to cultured representatives.. Kinetoplastids, including presumably novel representatives appear to be specialists of halocline environments in the Eastern Mediterranean, and to comprise a significant fraction of the protist communities in the brines and haloclines of several basins. Article Title: Microbial eukaryote life in the new hypersaline deep-sea basin Thetis. Article Snippet: Only recently, a novel anoxic hypersaline (thalassic) basin in the eastern Mediterranean was discovered at a depth of 3,258 m. The halite-saturated brine of this polyextreme basin revealed one of the highest salt concentrations ever reported for such an environment (salinity of 348%).. Using a eukaryote-specific probe and fluorescence in situ hybridization, we counted 0.6 9 10 protists per liter of anoxic brine.. SSU rRNA sequence analyses, based on amplification of environmental cDNA identified fungi as the most diverse taxonomic group of eukaryotes in the brine, making deep-sea brines sources of unknown fungal diversity and hotspots for the discovery of novel metabolic pathways and for secondary metabolites. Article Title: A molecular approach to identify active microbes in environmental eukaryote clone libraries. Article Snippet: A rapid method for the simultaneous extraction of RNA and DNA from eukaryote plankton samples was developed in order to discriminate between indigenous active cells and signals from inactive or even dead organisms.. The method was tested using samples from below the chemocline of an anoxic Danish fjord.. The simple protocol yielded RNA and DNA of a purity suitable for amplification by reverse transcription-polymerase chain reaction (RT-PCR) and PCR, respectively. Article Title: Microbial eukaryotes in the hypersaline anoxic L'Atalante deep-sea basin. Article Snippet: Eva Alexander,1 Alexandra Stock,1 Hans-Werner Breiner,1 Anke Behnke,1 John Bunge,2 Michail M. Yakimov3 and Thorsten Stoeck1* University of Kaiserslautern, School of Biology, Erwin-Schroedinger-Strasse 14, D-67773, Kaiserslautern, Germany.. Department of Statistical Science, Cornell University, Ithaca, NY 14853, USA.. Institute for Coastal Marine Environment, IAMC-CNR, Spianata S. Raineri, 86, 98122 Messina, Italy. Polymerase Chain Reaction:Article Title: Novel active kinetoplastids associated with hypersaline anoxic basins in the Eastern Mediterranean deep-sea Article Snippet: The combination of nearly saturated salt concentration and corresponding high density, high hydrostatic pressure, absence of light, anoxia, and a sharp chemocline make the deep hypersaline anoxic basins in the Eastern Mediterranean Sea some of the most polyextreme habitats on Earth.. Using kinetoplastid-specific primers, we detected kinetoplastid flagellates in some of the harshest deep-sea environments known to date, including some whose small subunit ribosomal RNA gene sequences are not closely related to cultured representatives.. Kinetoplastids, including presumably novel representatives appear to be specialists of halocline environments in the Eastern Mediterranean, and to comprise a significant fraction of the protist communities in the brines and haloclines of several basins. Article Title: Microbial eukaryote life in the new hypersaline deep-sea basin Thetis. Article Snippet: Only recently, a novel anoxic hypersaline (thalassic) basin in the eastern Mediterranean was discovered at a depth of 3,258 m. The halite-saturated brine of this polyextreme basin revealed one of the highest salt concentrations ever reported for such an environment (salinity of 348%).. Using a eukaryote-specific probe and fluorescence in situ hybridization, we counted 0.6 9 10 protists per liter of anoxic brine.. SSU rRNA sequence analyses, based on amplification of environmental cDNA identified fungi as the most diverse taxonomic group of eukaryotes in the brine, making deep-sea brines sources of unknown fungal diversity and hotspots for the discovery of novel metabolic pathways and for secondary metabolites. Article Title: A molecular approach to identify active microbes in environmental eukaryote clone libraries. Article Snippet: A rapid method for the simultaneous extraction of RNA and DNA from eukaryote plankton samples was developed in order to discriminate between indigenous active cells and signals from inactive or even dead organisms.. The method was tested using samples from below the chemocline of an anoxic Danish fjord.. The simple protocol yielded RNA and DNA of a purity suitable for amplification by reverse transcription-polymerase chain reaction (RT-PCR) and PCR, respectively. Article Title: Microbial eukaryotes in the hypersaline anoxic L'Atalante deep-sea basin. Article Snippet: Eva Alexander,1 Alexandra Stock,1 Hans-Werner Breiner,1 Anke Behnke,1 John Bunge,2 Michail M. Yakimov3 and Thorsten Stoeck1* University of Kaiserslautern, School of Biology, Erwin-Schroedinger-Strasse 14, D-67773, Kaiserslautern, Germany.. Department of Statistical Science, Cornell University, Ithaca, NY 14853, USA.. Institute for Coastal Marine Environment, IAMC-CNR, Spianata S. Raineri, 86, 98122 Messina, Italy. |